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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Predifferentiated amniotic fluid mesenchymal stem cells enhance lung alveolar epithelium regeneration and reverse elastase-induced pulmonary emphysema

Fig. 6

Transplantation of LEPLCs enhanced the expression of lung epithelial progenitor-like markers in the PPE-induced pulmonary emphysema mouse model. a Quantitative real-time RT-PCR and b Western blots analysis expression of SPC and AQP-5 in whole lung tissues of animals that received PBS, AFMSCs, or LEPLCs after 4-week period of PBS, AFMSCs, or LEPLCs treatment following PPE administration. Values were normalized to the Gapdh values and were expressed relative to the PBS group. The histogram on the right shows the semiquantitative densitometry of the Western blot analysis determined using ImageJ. Data are expressed as the mean ± SD; n ≥ 5 per group. *p < 0.05, **p < 0.01, and ***p < 0.001. c, d Immunofluorescence staining of (c) GFP-positive cells (green) and type II alveolar epithelial markers, pro-SPC (red); d type I alveolar epithelial markers, AQP-5 (green), and nucleus (DAPI; blue) in mouse lung tissues from each group treated as described above. The white dotted line areas in the (c) upper panel are magnified in the lower panel with 10 μm bars, and the expressions of pro-SPC and GFP were co-localized (yellow, arrowheads). Scale bar = 100 μm

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