Fig. 1From: Characterization of spontaneous spheroids from oral mucosa-derived cells and their direct comparison with spheroids from skin-derived cellsCharacterization of spontaneous spheroids from oral mucosa-derived cells. a Morphological changes of oral mucosa-derived cells on the specialized culture dish. In serum-free culture medium and serum-free culture medium with bFGF, EGF, and B27 supplement, no spheroid formation was observed. Only cells in medium containing serum formed spheroids spontaneously, which were then maintained. Scale bars = 100 μm. b Immunofluorescence analysis of Sox2, SSEA1, Oct4, and Nanog expression in spheroids from oral mucosa-derived cells. A positive reaction was observed in almost all spheroid-forming cells. c The expression of Nestin in spheroids from oral mucosa-derived cells. Scale bars = 50 μm. DAPI, 4′,6-diamidino-2-phenylindoleBack to article page