Fig. 6

Neurogenic induction of spheroid-derived cells from oral mucosa and skin. a Immunofluorescence images. βIII-tubulin- and MAP 2-positive cells were not observed, while Nestin-, NeuN-, Sox2-, and S100β-positive cells were found in oral mucosa cells (two-dimensional culture). Nestin-, βIII-tubulin-, MAP 2-, NeuN-, Sox2-, and S100β-positive cells were observed in the spheroid-derived cells from both oral mucosa and skin. b The expression levels of neural cell markers were analyzed using qRT-PCR. The expression levels of MAP 2, MBP, Nestin, and Nurr1 in spheroid-forming cells from the oral mucosa were significantly higher than those in spheroid-forming cells from the skin. c Expression of MAP 2, MBP, and Nestin in the spheroid-forming cells from the oral mucosa and CBDCs. The expression levels of MAP 2, MBP, and Nestin in the spheroid-forming cells from oral mucosa were significantly higher than those in spheroid-forming cells from CBDCs. The data are presented as the mean ± SEM; n = 3. *p < 0.05; **p < 0.01; ***p < 0.001. Scale bars = 50 μm. DAPI, 4′,6-diamidino-2-phenylindole