Fig. 6

miR-34a overexpression enhanced bone formation in irradiated bone defects. a 2D and 3D micro-CT images of bone formation in the defect area of irradiated rats at 8 weeks; scale bar of CT 2D images = 1.5 mm. b Representative H&E staining of new bone formation; scale bar = 1 mm. c Sequential fluorescent labeling of bone formation and mineralization; Alizarin Red S (AL), Calcein (CA), Tetracycline Hydrochloride (TE); scale bar = 1 mm. d The morphometric analysis of BV/TV for the micro-CT images (n = 5). e The quantitative results of sequential fluorescent labeling (n = 3). Data are shown as mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001