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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Deferasirox drives ROS-mediated differentiation and induces interferon-stimulated gene expression in human healthy haematopoietic stem/progenitor cells and in leukemia cells

Fig. 6

Effect of STAT1 inhibition on DFX-mediated differentiation in Kasumi-1 and K562 cells. a Western blot analysis of total STAT1 and pSTAT1tyr701 on whole protein Kasumi-1 (left) and K562 (right) cell extracts. Cells were treated with 100 μM DFX for 24 h ± a pre-incubation with 1 μM fludarabine (FLUDA) for 1 h. Per cell type, representative blots and bar histograms of densitometric analysis showing mean ± SEM p-STAT1/STAT1 ratio of three independent experiments are shown (*p < 0.05 vs CTRL; **p < 0.05 vs DFX). b Percentage of expression-inhibition of IFN-related gene transcript levels in Kasumi-1 and K562 cells treated with 100 μM DFX for 24 h + a pre-incubation with 1 μM fludarabine (FLUDA) for 1 h, compared to 100 μM DFX only treatment (*p < 0.05 vs DFX-only-treated cells). c, e Cytofluorimetric analysis of CD36 (left) and CD14 (right) expression in Kasumi-1 cells evaluated as percentage of positive cells (c) and as mean of fluorescence intensity (MFI) (e). Bars represent the mean ± SEM of three independent experiments (*p < 0.05 vs CTRL). d, f Cytofluorimetric analysis of CD36 (left) and CD71 (right) expression in K562 cells evaluated as percentage of positive cells (d) and as mean of fluorescence intensity (MFI) (f). Bars represent the mean ± SEM of three independent experiments (*p < 0.05 vs CTRL)

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