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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Generation of functional dopaminergic neurons from human spermatogonial stem cells to rescue parkinsonian phenotypes

Fig. 4

Activation of proneurogenic factors is necessary for DA lineage specification. a Quantitative RT-PCR analysis of genes essential for pro-neurogenesis DA lineage specification at the indicated induction time (1, 2, and 3 weeks). b Immunofluorescence revealed the expression of these indicated molecules in TH-positive cells induced by this special condition. c The yield of DA neurons with prolonged culture time. All data are reported as the means ± SEM. “*” and “**” represent p < 0.05 and p < 0.01, respectively, vs corresponding controls. Three independent experiments are represented. Scale bars = 10 μm

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