Fig. 6
Physiological properties of neurons derived from hSSCs. a Functional characterization of the L-type calcium channel. The panels show typical calcium response imagines of hSSC-derived cells at 2–3 weeks postinduction.1F/F0 represents the ratio of fluorescence intensity of cells for 0 s and at the indicated time. BayK (10 mM) in the presence or absence of nifedipine (5 mM) was administered at the indicated time point. b–d Representative photographs of Ca2+ imaging of differentiated neurons or hSSCs in the presence or absence of nifedipine after treatment with BayK. Approximately 50 cells were tested in each experiment; the representative results are shown. Scale bar = 50 μm. e Current-clamp recordings of action potentials evoked in SSC-derived neurons after 3 weeks of induction. Action potentials were suppressed by TTX. f Voltage-clamp recording of hSSC-derived neurons. Depolarized sodium and potassium currents were evoked by the membrane potential to different levels. g Sodium and potassium currents were attenuated by sodium and potassium channel inhibitors TTX (400 nm) and TEA (500 nm), respectively