Generation of functional dopaminergic neurons from human spermatogonial stem cells to rescue parkinsonian phenotypes

Table 1 Primer sequences used for qPCR

Gene	Forward primer	Reverse primer	Product size (bp)	Tm (°C)
Tuj-1	GGCCAAGGGTCACTACACG	GCAGTCGCAGTTTTCACACTC	85	62
TH	GGGCTGTGTAAGCAGAACG	AAGGCCCGAATCTCAGGCT	107	61
DAT	TTTCTCCTCTCCGTCATTGGC	TGAAGCCCACACCTTTCAGTAT	226	62
Nurr1	ACCACTCTTCGGGAGAATACA	GGCATTTGGTACAAGCAAGGT	175	61
GFAP	CTGCGGCTCGATCAACTCA	TCCAGCGACTCAATCTTCCTC	209	61
CNP	AACAAGGCTTCTCCCGAAAA	GTCTTCACTCTAGCAGCGT	149	62
Nestin	CTGCTACCCTTGAGACACCTG	GGGCTCTGATCTCTGCATCTAC	141	62
CD133	AGTCGGAAACTGGCAGATAGC	GGTAGTGTTGTACTGGGCCAAT	99	62
Pax6	TGGGCAGGTATTACGAGACTG	ACTCCCGCTTATACTGGGCTA	111	61
GPR125	GCGTCATTACGGTCTTTGGAA	ACGGCAATTCAAGCGGAGG	199	60
Plzf	GAGATCCTCTTCCACCGCAAT	CCGCATACAGCAGGTCATC	139	61
DAZL	GCCCACAACCACGATGAATC	CGAGGTACAACATAGCTCCTTT	163	61.5
GFRA1	CCAAAGGGAACAACTGCCTG	CGGTTGCAGACATCGTTGGA	121	61.5
UCHL1	CCTGTGGCACAATCGGACTTA	CATCTACCCGACATTGGCCTT	201	62
EN-1	CGCAGCAGCCTCTCGTATG	CCTGGAACTCCGCCTTGAG	174	62.2
Pitx3	CAGAGGACGGTTCGCTGAAAA	AGCTGCCTTTGCATAGCTCG	240	62.7
Foxa2	GGAGCAGCTACTATGCAGAGC	CGTGTTCATGCCGTTCATCC	83	61.9
Lmax1a	ACGTCCGAGAACCATCTTGAC	CACCACCGTTTGTCTGAGC	248	61.5
Lmax1b	CGGACTGCGCCAAGATGTT	TTGACTCGCATCAGGAAGCG	160	62.5
OTX2	TATCTAAAGCAACCGCCTTACG	GCCCTAGTAAATGTCGTCCTC	134	60.2

ISSN: 1757-6512