Fig. 4

Inhibition of Hh signaling decreased the follicle number and FGSC proliferation. a HE staining shows a dramatic drop in primary follicle number after treatment with GANT61, the scale is 20 μm. b Dual-IF detects coexpression of Oct4 and Mvh, bar is 20 μm. c, d IHC of Mvh and Oct4, individually, the scale is 50 μm. e The number of cells positive for Mvh and Oct4 and double positive for Mvh/Oct4. f mRNA expression levels of Mvh and Oct4. g, h Protein expression of Mvh and Oct4. *p < 0.05 and **p < 0.01