Fig. 6

Suppressing Hh pathway activity reduced the antioxidative stress ability of FGSCs and induced apoptosis. Measurement of GPx (a) and SOD2 (c) enzyme activity. Levels of GSH (b) and MDA (d) in FGSCs. e, f Intracellular ROS levels detected by DHR, the scale is 20 μm. g mRNA expression levels of Bcl-2, Bax, P16, and P21. h, i Protein expression levels of Bcl-2, Bax, P16, and P21 in FGSCs. j, k mRNA and protein ratios of Bcl-2 to Bax expression levels. l Detection of Caspase-3 enzyme activity in FGSCs. m. n Change in mitochondrial membrane potential levels by JC-10 probe detection, the scale is 20 μm. *p < 0.05 and **p < 0.01