Table 1 Primer sequences used for quantitative real-time PCR
Genes | Primer Sequence(5′-3′) | Gene number |
|---|---|---|
Gli1 | Forward: CCCAATACATGCTGGTGGTG Reverse: GCAACCTTCTTGCTCACACA | NM_010296.2 |
PTCH1 | Forward: TGTGCGCTGTCTTCCTTCTG Reverse: ACGGCACTGAGCTTGATTC | NM_001328514.1 |
CyclinD1 | Forward: GATGAGGAAGAGTTGCTAGAAGAG Reverse: TCGTCAGCCAATCGGTAGTAG | NM_007631.2 |
Mvh | Forward: GTGTATTATTGTAGCACCAACTCG Reverse: CACCCTTGTACTATCTGTCGAACT | NM_001145885.1 |
Oct-4 | Forward: AGCTGCTGAAGCAGAAGAGG Reverse: GGTTCTCATTGTTGTCGGCT | NM_013633.3 |
Stella | Forward: CCCAATGAAGGACCCTGAAAC Reverse: AATGGCTCACTGTCCCGTTCA | NM_139218.1 |
Fragilis | Forward: CTGGTCCCTGTTCAATACACTCTT Reverse: CAGTCACATCACCCACCATCTT | NM_025378.2 |
Nanog | Forward: TCTCCTCGCCCTTCCTCTGA Reverse: TCCGCATCTTCTGCTTCCTG | NM_001289828.1 |
Bcl-2 | Forward: GAACTGGGGGAGGATTGTGG Reverse: GCATGCTGGGGCCATATAGT | NM_009741.5 |
Bax | Forward: GAACCATCATGGGCTGGACA Reverse: AGCCACCCTGGTCTTGGAT | NM_007527.3 |
P16 | Forward: AAGGCTTTGAGAGCCCATCT Reverse: CACTTCTGTGAACGGTGTCC | NM_001040654.1 |
P21 | Forward: TAGAGAGCTGGATGCCACTG Reverse: CCTGGCATAGCCAGTGTAGA | NM_001111099.2 |
GAPDH | Forward: CGTGCCGCCTGGAGAAACCTG Reverse: AGAGTGGGAGTTGCTGTTGAAGTCG | NM_001289726.1 |