Fig. 2

Feline ASCs decrease activated PBMC viability and induce cell cycle arrest in activated T lymphocytes. Representative images of flow cytometric analysis on day 4 from 5 MLR experiments with condition of a PBMCs only, b mitogen-activated PBMCs, and c PBMCs in co-incubation with feline ASCs. d the percentage of viable PBMCs decreased after mitogen activation (p = 0.04) and was further exacerbated by the co-incubation with feline ASCs (p = 0.008). Flow cytometric scatter plot of cell cycle analysis on T lymphocyte DNA content (7-AAD) and proliferation determined through BrdU incorporation of e PBMCs only, f mitogen-activated PBMCs, and g PBMCs in co-incubation with feline ASCs. h Percentage of T cells in the apoptotic, G0–G1, S, and G2–M phases from cell cycle analysis revealed that the percentage of T lymphocytes in the G0–G1 phase increased with a concurrent decrease in the S-phase upon co-incubation with feline ASCs (p = 0.03). *p < 0.05 **p < 0.01