Fig. 3

PGE2 secretion partially mediates feline ASC inhibition of T lymphocyte proliferation. a Measurement of iNOS production from supernatant of MLR on day 4 of co-incubation. Data from 5 independent experiments. b Measurement of IDO production from supernatant of MLR on day 4 of feline ASC and PBMC co-incubation with and without transwell. Data from 5 independent experiments. Degree of proliferation in the MLR with inhibition of soluble mediators: c addition of TGF-β and IFN-γ blocking antibody, d blocking of PGE2 with indomethacin, e blocking IDO with 1-methyltryptophan and PGE2 with indomethacin. Percentage of proliferation is normalized to 100% on mitogen activated condition for comparability. Blocking PGE2 significantly hindered the degree of suppression (p = 0.03). *p < 0.05, **p < 0.01