Fig. 2

Characterization of PC-iPS and PEF-iPS cells. a Immunocytochemistry analysis of PC-iPS and PEF-iPS cells, scale bar 10 μm. b Karyotype analysis of PC-iPS and PEF-iPS cells. c EB formation of PC-iPS and PEF-iPS cells, scale bar 200 μm. d Immunohistochemistry of 3 germ layers differentiation of PC-iPS and PEF-iPS cells in vitro, scale bar 100 μm. e Toggling between dome- and flattened disc-shaped PC-iPS cells, scale bar 200 μm. f RT-PCR analysis of dome- and flattened disc-shaped PC-iPS cells (****p < 0.0001, ***p < 0.001, **p < 0.5; ns, not significant)