Fig. 5
Analysis GFP PC-iPS contribution to chimera mouse in vivo. a Representative bright-field and fluorescence images of chimeric mouse fetuses. b Flow cytometry analysis of GFP positive cells of chimeric mouse fetuses. c Nested PCR of GFP and pig mtDNA of chimeric mouse fetuses (the GFP-labeled PC-iPS cells’ DNA and PB513B-1 plasmid were used as GFP sequence positive controls; the GFP-labeled PC-iPS cells’ DNA and PC DNA were used as pig mtDNA sequence positive control; double distilled water (ddH2O) and mouse DNA were used as negative controls). d Representative bright-field and fluorescence images of extraembryonic tissues. e Flow cytometry analysis of GFP-positive cells within the extraembryonic tissue. f Nested PCR of GFP and pig mtDNA of chimeric mouse extraembryonic tissues. g Representative bright-field and fluorescence images of degenerated mouse embryo, scale bar 500 μm