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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Correlation between frataxin expression and contractility revealed by in vitro Friedreich’s ataxia cardiac tissue models engineered from human pluripotent stem cells

Fig. 6

FXN expression rescue of FRDA hESC-hvCTS model. a FXN (i) transcript and (ii) protein expression (normalized to GAPDH expression) and (iii) representative Western blot image of hESC-hvCTS double-transduced with Lv-shFXN and Lv-FXN relative to Lv-shFXN and Lv-RFP control (n = 5–6). Transcript and protein data were generated from 5 to 6 independent batches of cell differentiation, respectively. b Representative contractile force traces from isometric force measurement relative to % Lmax for hESC-hvCTS double-transduced with Lv-shFXN and Lv-FXN, relative to Lv-shFXN and Lv-RFP control. c Developed force, contractile rate, and relaxation rate for % Lmax measured at 1 Hz stimulation using isometric force measurement for hESC-hvCTS double-transduced with Lv-shFXN and Lv-FXN, relative to Lv-shFXN and Lv-RFP control (Lv-shFXN+Lv-FXN: n = 16; Lv-shFXN+Lv-RFP: n = 11). Data were generated by 4 independent differentiation batches. Data are shown as mean ± SEM. Statistical significance indicated by *p < 0.05 and **p < 0.01

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