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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Mesenchymal stem cell repression of Th17 cells is triggered by mitochondrial transfer

Fig. 2

Mitochondrial transfer from BM-MSCs significantly reduces IL-17 production independently of TNF-α/IFN-γ stimulation. a Representative dot plots of FOXP3 and IL-17 expression (left panels) in Th17 cells after 3 days of activation by incubation with beads coupled to anti-CD3/CD28 antibodies following (+) or not (−) co-culture with BM-MSCs for 4 or 24 h. Graphs (right panels) show the quantification of the results. b BM-MSC mitochondrial transfer to Th17 cells after 4 and 24 h of co-culture. BM-MSCs were pre-incubated or not (Basal) with TNF-α and IFN-γ. Mitochondrial transfer is expressed as the percentage of MitoTracker-positive T cells relative to all T cells and as the mean fluorescence intensity (MFI). c IL-17 and FOXP3 expression quantification in Th17 cells co-cultured or not (None) with BM-MSCs pre-incubated or not (Basal) with TNF-α and IFN-γ. d Proliferation of Th17 cells after 3 days of activation by incubation with beads coupled to anti-CD3/CD28 antibodies following co-culture or not (None) with BM-MSCs pre-activated or not (Basal) with TNF-α and IFN-γ. Data are the mean ± SEM of at least 3 independent experiments using PBMCs from five donors and BM-MSCs from three donors (MSC to T cell ratio = 1:25)

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