Skip to main content
Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Mesenchymal stem cell repression of Th17 cells is triggered by mitochondrial transfer

Fig. 3

Mitochondrial transfer from BM-MSCs significantly increases the generation of Treg cells. a Schematic representation of the cell sorting experiment. Th17 cells were co-cultured with MitoTracker (Mito)-labeled BM-MSCs for 4 h, and then Mito+ and Mito Th17 cells were isolated by cell sorting. b Representative dot plots of cells with the Th17 and Treg phenotypes. c IL-17 expression quantification 24 h after cell sorting of Mito+ and Mito Th17 cells by flow cytometry. d CD25 and FOXP3 expression quantification 24 h after sorting of Mito+ and Mito cells by flow cytometry. Data are the mean ± SEM of 3 independent experiments using PBMCs from four donors and BM-MSCs from three donors (MSC to T cell ratio = 1:25)

Back to article page