Fig. 5

Artificial transfer of BM-MSC mitochondria in Th17 cells significantly reduces IL-17 production independently of TNF-α/IFN-γ activation. a Representative histogram showing MitoTracker-positive Th17 cells (flow cytometry) 24 h after artificial mitochondrial transfer using the MitoCeption protocol. Mitochondria were isolated from MitoTracker-labeled BM-MSCs pre-activated or not with TNF-α/IFN-γ. b Quantification of artificial mitochondrial transfer in Th17 cells as the percentage of MitoTracker-positive Th17 cells (left) or MFI (right). c Representative image of BM-MSC mitochondria after MitoCeption (ratio 0.025) in Th17 cells. d Quantification of IL-17 production by Th17 cells after 3 days of activation with beads coated with anti-CD3/CD28 antibodies following MitoCeption or not (None) of mitochondria isolated from BM-MSCs pre-activated or not (Basal) with TNF-α and IFN-γ. e FOXP3 expression quantification in Th17 cells after 3 days of activation with beads coated with anti-CD3/CD28 antibodies following MitoCeption or not (None) of mitochondria isolated from BM-MSCs pre-activated or not (Basal) with TNF-α and IFN-γ. f Proliferation of Th17 cells after 3 days of activation with beads coated with anti-/CD28 antibodies following MitoCeption or not (None) of mitochondria isolated from BM-MSCs pre-activated or not (Basal) with TNF-α and IFN-γ. Data are the mean ± SEM of at least 3 independent experiments using PBMCs from five donors and BM-MSCs from three donors and a ratio for MitoCeption of 1 BM-MSC:25 Th17 cells