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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Interferon gamma inhibits the differentiation of mouse adult liver and bone marrow hematopoietic stem cells by inhibiting the activation of notch signaling

Fig. 1

IFN-γ inhibits the colony-forming activity of HSCs of liver and bone marrow. Hematopoietic colony formation of the mononuclear cells (a, c) or LSK cells (e, g) from the adult liver (a, e) or bone marrow (c, g) (the picture shows a single colony in a well of 24-well cell culture plate). A total of 1 × 105 bone morrow or liver mononuclear cells were freshly isolated from adult C57BL/6j mice, and 1 × 103 bone marrow or liver LSK cells were sorted by FACS and plated into complete methylcellulose medium, with or without IFN-γ stimulation, and incubated for 10 to 14 days. The number of colonies (≥ 50 cells were defined as one clone) was counted under an inverted phase contrast microscope. GM-CFU: the added cytokines included SCF, IL-3, FLT-3-L, IL-7, and GM-CSF. M-CFU: the added cytokines included SCF, IL-3, FLT-3-L, IL-7, and M-CSF. Picture original magnification: × 200 (a, c, e, and g). Scale bar, 200 μm. Statistical analysis for the number of GM-CFUs and M-CFUs from MNCs or LSK cells of the BM and liver (n = 3–6) (b, d, f, and h). All colonies were counted in a well of 24-well cell culture plate. Bars represent the mean ± SEM of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001

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