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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Interferon gamma inhibits the differentiation of mouse adult liver and bone marrow hematopoietic stem cells by inhibiting the activation of notch signaling

Fig. 2

Knockout of IFN-γ promotes the proliferation ability of LSKs from liver and BM. Hematopoietic colony formation of LSK cells (a, b) from the adult liver (a) or bone marrow (b) (the picture shows a single colony in a well of 24-well cell culture plate). FACS was used to sort 1 × 103 bone marrow or liver LSK cells from adult C57BL/6j mice and GKO mice and plated into complete methylcellulose medium with or without IFN-γ stimulation and incubated for 10 to 14 days. The number of colonies (≥ 50 cells were defined as one clone) was counted under an inverted phase contrast microscope. GM-CFU: the added cytokines included SCF, IL-3, FLT-3-L, IL-7, and GM-CSF. M-CFU: the added cytokines included SCF, IL-3, FLT-3-L, IL-7, and M-CSF. Picture original magnification: × 200 (a, b). Scale bar, 200 μm. Statistical analysis for the number of GM-CFUs and M-CFUs from LSK cells of the BM and liver (n = 3–6) (c, d). All colonies were counted in a well of a 24-well cell culture plate. Bars represent the mean ± SEM of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001. The proportion of Lin c-kit+ sca-1+ (LSK) cells and LT-HSC, ST-HSC, and MPP cells gated from LSK cells and BrdU gated from LT-HSCs in the flow plot from the adult C57BL/6j mice and GKO mouse livers or BM (n = 5–10) (e, f). Statistical analysis of the percentage of BrdU cells gated from the LT-HSCs in the adult mouse liver and BM (g). Bars represent the mean ± SEM of three independent experiments. *p < 0.05

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