Fig. 5

IFN-γ impacts the differentiation rate from LSK cells to T progenitor cells and γδT cells. a The proportion of LSK cells and T progenitor cells (DN1, DN2, DN3, and DN4 cells) in the flow plot was gated from Lineage⁻ cells and the expression of Ki67 on total LSK cells and DN cells after the adult mice liver or BM LSK cells were co-cultured with OP9-DL1 cells, with or without IFN-γ stimulating at days 7 (n = 5–10). b The expression of Ki67 on total LSK cells and DN cells after the adult mouse liver or BM LSK cells were co-cultured with OP9-DL1 cells, with or without IFN-γ stimulation at day 0 and day 7 (n = 5–10). c The proportion of γδT cells and αβT cells in the flow plot after the adult C57BL/6j mice liver or BM LSK cells were co-cultured with OP9-DL1 cells, with or without IFN-γ stimulating at day 21. d Apoptosis levels of liver LSK cells and BM LSK cells in the co-cultured system were detected using 7-AAD at day 21