Fig. 7

Notch signaling impacts the differentiation of liver and BM hematopoietic stem cells. a Six-week-old male mice were given a gavage dose of GSI and DMSO for 1 week, and the gene expression level of HES1 and HES5 on the LSK cells of the liver in the GSI-treated mice and the control mice were detected. b The proportions of LSK and LT-HSC, ST-HSC, and MPP cells in the flow plot were gated from LSK cells from the GSI-treated mice and the control mice livers or BM (n = 5–10). c The proportion of CLP cells in the flow plot was gated from Lin⁻ IL-7Rα⁺ cells from the GSI-treated mice and the control mice livers or BM (n = 5–10). Bars represent the mean ± SEM of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001