Fig. 3

Stem cell marker expression in the BMSCs examined by immunostaining. a–c The cells were stained with nucleostemin. d–f The cells were stained with stro-1. g–i The cells were stained with CD44. j–l The cells were stained with CD90. a, d, g, j The cells were stained with H33342. b, e, h, k The cells were stained with the antibodies. c, f, i, l The images were merged images by a, d, g, j, and b, e, h, k. m The semi-quantification of positively stained cells by each stem cell marker. The insert images were enlarged box areas in the images of b and c. Bars 100 μm