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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: High-content screen in human pluripotent cells identifies miRNA-regulated pathways controlling pluripotency and differentiation

Fig. 2

Effects of miRNAs on nuclear OCT4 levels in H1 hESCs and NT2 cells. a H1 hESCs and NT2 cells were transfected with miR mimics and the negative control miR-Ctr (PMC) and then submitted to quantitative fluorescence microscopy. OCT4 nuclear median intensity is represented as a percentage relative to the value observed in cells transfected with PMC (NT2 cell depicted as triangles and H1 hESCs as circles). Colored bars in the bottom of the figure indicate the observed change in miRNA expression in H1 and NT2 cells, following induction of differentiation [10]. b Graphical representation of selected primary transcripts of miR clusters, with the relative position of the pre-mirs and of the mature miRs. Mature miRs are represented as colored boxes in the 5′ (-5p miR) or 3′ (-3p miR) side of the corresponding pre-mir. Each miR family is represented with a distinct color. Families were defined, as represented in the TargetScan 7 database. For simplicity, only the first representative miR of the family was used in the figure legend. Additional information regarding families can be found in Table S1 (in Additional file 2). Boxes with dotted lines indicate miRs that were not evaluated in our study

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