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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: The role of insulin as a key regulator of seeding, proliferation, and mRNA transcription of human pluripotent stem cells

Fig. 2

The effect of insulin on pluripotency phenotype and expansion of hiPSC colonies. hiPSC colonies maintain both their pluripotent. Morphology by phase contrast (a) and expression of key pluripotency markers, SOX2, OCT4 (also known as POU5F1), and TRA-1-60 (b) after 3 days of insulin starvation. Scale bar, 100 μM. Whole-well images (each cell line in triplicates) were taken after crystal violet staining (c) and quantification of surface area covered by hiPSC (d). The number of cells (each cell line in triplicates) in each culture condition was determined through cell count (e). Data represented as mean ± SD. Two-tailed Student’s t test was used for comparison of groups

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