Fig. 2

CCDC144NL-AS1 knockdown endows human PSCs with naïve-like dome-shaped clusters. a Bright-field (left) and fluorescence (right) images of H9 cells infected with sh-CCDC144NL-AS1 or sh-NC viruses on day 4. ShRNA expression plasmids used in this study contain an EGFP expression element, so EGFP fluorescence intensity reflects the virus infection efficiency of cells. Scale bars, 100 μm. b Bright-field (left) and fluorescence (right) images of H9 cells infected with sh-CCDC144NL-AS1 or sh-NC viruses on day 8. Scale bars, 100 μm. c Representative images of stable CCDC144NL-AS1-KD-H9 and NC-H9 cells. Scale bars, 100 μm. d Five representative confocal EGFP fluorescence images capturing the rotation process of CCDC144NL-AS1-KD-H9 or NC-H9 colonies. Scale bars, 50 μm. The values of rotation angles are indicated above the images. e Representative images of CCDC144NL-AS1-KD-H14, NC-H14 cells, CCDC144NL-AS1-KD-HDF-iPS, and NC-HDF-iPS cells. Scale bars, 100 μm. f Quantitative RT-PCR analyses of CCDC144NL-AS1’s expression in CCDC144NL-AS1-KD hPSCs and their control cells. Error bars indicate SEM (n = 3). *p < .05; **p < .01; ***p < .001