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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Long noncoding RNA CCDC144NL-AS1 knockdown induces naïve-like state conversion of human pluripotent stem cells

Fig. 3

Naïve-like pluripotency validation of CCDC144NL-AS1-downregulated human ESCs. Quantitative RT-PCR analyses of three core pluripotency genes (a), lineage commitment factor genes (b), and naïve pluripotency-associated genes (c) in CCDC144NL-AS1-KD-H9 and NC-H9 cells. Error bars indicate SEM (n = 3). *p < .05; **p < .01; ***p < .001. Immunostaining images of pluripotency-associated markers OCT4, NANOG, SOX2, SSEA3/4, and TRA-1-60 (d) and naïve pluripotency-specific marker TFCP2L1 (e). Scale bars, 75 μm. f Western blot detection of naïve pluripotency-related transcription factors, such as NANOG and KLF4, and shared pluripotency transcription factors, such as OCT4 and SOX2, in CCDC144NL-AS1-KD-H9 and NC-H9 cells. β-Tubulin was used as an endogenous control. g Single-cell cloning efficiencies of CCDC144NL-AS1-KD-H9 and NC-H9 cell lines. Error bars indicate SEM (n = 3); ***, p < .001. h Growth curves of CCDC144NL-AS1-KD-H9 and NC-H9 cell lines. Error bars indicate SEM (n = 3). *p < .05; **p < .01; ***p < .001. i Karyotype analysis of CCDC144NL-AS1-KD-H9 and NC-H9 cell lines

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