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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Long noncoding RNA CCDC144NL-AS1 knockdown induces naïve-like state conversion of human pluripotent stem cells

Fig. 4

Developmental potential of CCDC144NL-AS1-downregulated human ESCs in vitro and in vivo. Quantitative RT-PCR analyses of expression levels of lineage-specific genes in CCDC144NL-AS1-KD-H9- and NC-H9-derived neuronal (a), endodermal (c), and mesodermal (e) cells. Error bars indicate SEM (n = 3). *p < .05; **p < .01; ***p < .001. Western blot analyses of lineage-specific genes’ expression in CCDC144NL-AS1-KD-H9- and NC-H9-derived neuronal (b), endodermal (d), and mesodermal (f) cells. β-Tubulin was used as an endogenous control. g Morphologies of embryoid bodies differentiated from CCDC144NL-AS1-KD-H9 and NC-H9 cells. Scale bars, 100 μm. Quantitative RT-PCR analyses of lineage-related genes in embryoid bodies generated from CCDC144NL-AS1-KD-H9 (h) and NC-H9 (i) cells. Error bars indicate SEM (n = 3). *p < .05; **p < .01; ***p < .001. j Embryoid body numbers generated from per 106 CCDC144NL-AS1-KD-H9 and NC-H9 cells. Error bars indicate SEM (n = 3). **p < .01. k Hematoxylin and eosin staining of teratomas containing tissues of all three embryonic germ layers derived from CCDC144NL-AS1-KD-H9 and NC-H9 cells. (m, p) Gut-like epithelium (endoderm). (n, q) Cartilage (mesoderm). (o, r) Neural tissue (ectoderm). Scale bars, 500 μm. l Teratoma formation rate of CCDC144NL-AS1-KD-H9 and NC-H9 cells. Error bars indicate SEM (n = 13). **p < .01

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