Fig. 3From: In vivo tracking on longer retention of transplanted myocardin gene-modified adipose-derived stem cells to improve erectile dysfunction in diabetic ratsMyocardin promoted SRF and inhibited SOX2 and OCT4. The serum response factor (SRF) was elevated in mRNA (a) and protein levels (b) along with overexpression of myocardin. c The colocalization of Myocardin and SRF was detected in the nucleus under × 2000 magnification with confocal microscopy. The stem cell markers SOX2 and OCT4 were inhibited by myocardin at the mRNA (d) and protein levels (e), as well as the immunofluorescence intensities indicated by confocal microscopy under × 600 (upper row) and × 1200 (lower row) magnifications (f). The red rectangle indicates the zoomed area. Scale bar = 50 μm. Cell experiments performed n = 3. **p < 0.01; ***p < 0.001Back to article page