Fig. 2

a Significant constitutive transcription of HPRT, Thy1, SCF, c-kit, somatostatin (SST), VDR and vitamin D machinery enzymes (1α-Hydroxylase, 24-Hydroxylase and 25-Hydroxylase) out of a total panel of 15 genes were detected at normal culture conditions of ADMSC. b Western blot gel was loaded with 40 μg (lane 1) and 20 μg (lane 2) of ADMC lysate. The 54 kDa vitamin D receptor (VDR) protein was probed with a monoclonal rabbit anti-human antibody followed by goat anti-rabbit Ig-HRP secondary antibody. The membrane was incubated with enhanced chemiluminescence solution for 1 min and developed with Odyssey® imaging system (LICOR®, Germany). For PCR analysis, HPRT was considered as a housekeeping gene and fold change was achieved with 2^−∆∆Ct method. To compare the groups, one-way ANOVA with Turkey’s post hoc test was utilised