Fig. 3

Influence of increasing concentrations (50, 100, 150 nM) of vitamin D3 to 120 h culture of ADMSC on the expression of selected gene transcripts. a–d The influence of vitamin D3 on the gene expression levels of metabolising enzymes 1α-Hydroxylase, 24-Hydroxylase, 25-Hydroxylase and the VDR. The expression of 24-Hydroxylase was regulated by vitamin D3 compared to control without vitamin D3 (p < 0.001) while other target gene transcripts remained stable without changes. e Relative expression of somatostatin gene was modulated in decreasing fashion in the presence of vitamin D3 (p < 0.001). Data represent the mean ± SEM (n = 4 runs). To compare the different concentration, one-way ANOVA with Turkey’s post hoc test was utilised. Values considered significant *p < 0.05, **p < 0.01 and ***p < 0.001, compared with the absence of vitamin D3 as a control condition