Fig. 3

Calculated percent contributions of the variables toward the ALP expression. The variables had either a net positive or a net negative contribution to the ALP expression. If the (+) variability of the variable resulted in higher ALP expression, the contribution was positive; if it led to lower ALP expression, the contribution was deemed negative. The average experimental error between the three biological replicates (4.1%) was used as the significance limit, which resulted in the following ranking of the variables in terms of their final contribution: (1) BMP-9, (2) serum, (3) hASC source, (4) AP, (5) DEX, and (6) seeding density