Table 1 Target variables used for the screening of culture parameters for the osteogenic differentiation of hASCs. hASC source and seeding density were chosen as factors related to the stromal cells; base medium, serum, L-ascorbate-2-phosphate, ß-glycerophosphate, dexamethasone, and BMP-9 were chosen as factors related to the medium. The factor variability was set between a certain range based on the values commonly used in literature
Variable | Description | Range of variability from (-) to (+) | References | ||
|---|---|---|---|---|---|
V1 | hASC source | ZB | EFS | [14] | |
V2 | Cell seeding density, per well | 2000 | 6000 | 10000 | [5] |
V3 | Base culture medium | DMEM/F-12 | DMEM | [15] | |
V4 | Serum source | 5% hPL | 10% FBS | [8] | |
V5 | L-ascorbate-2-phosphate | 0 | 50 μM | [16] | |
V6 | ß-glycerophosphate | 0 | 10 mM | [16] | |
V7 | Dexamethasone | 0 | 100 nM | [16] | |
V8 | Bone morphogenetic protein-9 | 0 | 100 ng/mL | [18] | |