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Table 1 Target variables used for the screening of culture parameters for the osteogenic differentiation of hASCs. hASC source and seeding density were chosen as factors related to the stromal cells; base medium, serum, L-ascorbate-2-phosphate, ß-glycerophosphate, dexamethasone, and BMP-9 were chosen as factors related to the medium. The factor variability was set between a certain range based on the values commonly used in literature

From: Design of experiments to assess the effect of culture parameters on the osteogenic differentiation of human adipose stromal cells

Variable Description Range of variability from (-) to (+) References
V1 hASC source ZB EFS [14]
V2 Cell seeding density, per well 2000 6000 10000 [5]
V3 Base culture medium DMEM/F-12 DMEM [15]
V4 Serum source 5% hPL 10% FBS [8]
V5 L-ascorbate-2-phosphate 0 50 μM [16]
V6 ß-glycerophosphate 0 10 mM [16]
V7 Dexamethasone 0 100 nM [16]
V8 Bone morphogenetic protein-9 0 100 ng/mL [18]