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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Long-term culture of mesenchymal stem cells impairs ATM-dependent recognition of DNA breaks and increases genetic instability

Fig. 2

Effect of inhibition of ATM and DNA-PK on γH2AX- and 53BP1-foci formation in aging MSCs after exposure to irradiation. a One-week and b 8-week cultured MSCs were pre-incubated with DMSO and a chemical ATM (ATMi) or DNA-PK (DNA-PKi) inhibitor and irradiated with 6 Gy before γH2AX- and 53BP1-foci formation was detected 90 min post irradiation. Foci formation in at least 50 nuclei was automatically quantified using Keyence BZ-II Analyzer software, and the relative amount of repair foci was normalized to the 90 min value (mean values ± SEM, n = 3,)

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