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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Long-term culture of mesenchymal stem cells impairs ATM-dependent recognition of DNA breaks and increases genetic instability

Fig. 3

Kinetics of γH2AX- and 53BP1-foci formation in aging MSCs after exposure to 2 Gy. Aging MSCs were irradiated with 2 Gy before γH2AX- and 53BP1-foci formation was detected 90, 180, 300, and 420 min post irradiation. Foci formation in at least 50 nuclei was automatically quantified using Keyence BZ-II Analyzer software and the relative amount of repair foci was normalized to the 90 min value. Data plotted on a linear scale can be found in the Additional file 7: Figure S5. Mean repair half time in phase 1 and phase 2 and fraction of unrepaired DNA breaks is given in the Additional file 4: Table S1

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Stem Cell Research & Therapy

ISSN: 1757-6512