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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Long-term culture of mesenchymal stem cells impairs ATM-dependent recognition of DNA breaks and increases genetic instability

Fig. 4

Frequency of micronuclei after exposure to irradiation in aging MSCs in vitro. a MSCs were cultured for 1, 4, 8, and 12 weeks under hypoxia in vitro. Mean number of micronuclei per 100 binuclear MSCs was detected after sham-irradiation (0 Gy) and 0.05, 0.5, 2, and 6 Gy irradiation and incubation for 5 days to allow repair of DNA damage in the presence of cytochalasin B. b Representative images of MSCs 5 days after 6 Gy γ-irradiation and DAPI staining showing cells with 1, 2, and 4 micronuclei. c Mean percentage of irradiated binucleated MSCs with two or more micronuclei (mean values ± SEM, n = 3)

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Stem Cell Research & Therapy

ISSN: 1757-6512