Fig. 1
From: High temperature suppressed SSC self-renewal through S phase cell cycle arrest but not apoptosis
Heat shock treatment inhibits SSC proliferation. a The CD1 SSC line can be stably cultured in vitro. b The SSC surface marker proteins GFRα-1 and PLZF were expressed in in vitro-cultured SSCs. c Viability of SSCs 18 h after heat shock treatment. Heat shock treatment at 43 °C for 45 min or at 43 °C for 60 min significantly inhibited the proliferation of SSCs 18 h after treatment. d The viability of SSCs was significantly reduced as early as 2 h after 45 min of 43 °C heat shock treatment. e Two and 18 h after treatment, compared with the control group, the cell density in the 43 °C 45-min heat shock-treated group was significantly reduced. *P < 0.05 compared with the control group. Bar = 50 μm