Fig. 4
3D-ADSC1000 resisted to oxidative stress in vitro. a Qualitative analysis of the viability of 2D-ADSC 24 h after treatment with exogenous oxidant (TBHP) using live/dead imaging; magnification × 100, scale bar 100 μm. b Quantitative analysis of viability of 2D-ADSC 24 h after treatment with exogenous oxidant (TBHP) using CCK-8 assay. Data represent the mean ± SEM of three independent experiments. c Representative live/dead image showing the viability of 3D-ADSC1000 24 h after treatment with TBHP; magnification × 100, scale bar 100 μm. d Quantitative assessment of viability of 3D-ADSC1000 24 h after treatment with TBHP using CCK-8 assay. The graph represents mean ± SEM of three independent experiments. e Basal levels of ROS in 2D-ADSC and 3D-ADSC1000 determined by using DCFDA reagent (mean ± SEM, three independent experiments). f Representative blot for basal level expressions of HO-1 in 3D-ADSC and 2D-ADSC and quantitative evaluation of the HO-1 expression from three independent experiments using GelQuantNET software. Representative blots and quantitative expressions of the antioxidant enzymes g SOD2 and h catalase (mean ± SEM, three independent experiments). **p < 0.01 and ***p < 0.001