Fig. 7From: Mouse embryonic palatal mesenchymal cells maintain stemness through the PTEN-Akt-mTOR autophagic pathwayCharacteristics of autophagy in MEPM cells. a LC3-II immunofluorescence staining before differentiation and after osteogenic and adipogenic differentiation. Cell nuclei (blue) were counterstained with DAPI; cytoplasm (red) was stained with Evans blue; scale bar: 20 μm. b Western blot showing expression levels of LC3-I/II and p62 proteins before and after osteogenic differentiation; N = 3; *P < 0.05; **P < 0.01. c TEM image showing large amount of autophagosomes (red arrows) before osteogenic differentiation, which decreased sharply after osteogenic differentiationBack to article page