Fig. 5

MiR-100 suppressed inflammation via repression of mammalian target of rapamycin (mTOR). a KEGG pathway enrichment analysis for SHED-Exos. The X-axis represents the percentage of the total number of annotated miRNAs in this pathway, whereas the Y-axis indicates the name of a pathway. b mTOR protein expression was analyzed by western blotting. c Protein semi-quantitative analysis of mTOR. d mTOR mRNA expression was analyzed by RT-qPCR. Statistical analysis was performed by one-way ANOVA followed by Tukey’s test. e Chondrocytes were treated with rapamycin (10 nM) to inhibit mTOR. Statistical analysis was performed by Student’s t test. f MiR-100 expression levels were quantified by RT-qPCR. g The expression of inflammatory factors was quantified by RT-qPCR. Data are presented as mean ± SEM. Data with P values < 0.05 were considered significant; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.001