Fig. 6

mTOR mRNA is a direct target of miR-100. a Sequences of the predicted miR-100-5p target sequences in the 3′UTR of mTOR mRNA and its mutant containing nucleotide substitutions in the 3′UTR. b The luciferase reporter assay was conducted to test whether the mTOR 3′UTR contains a binding site for miR-100. Luciferase assays show decreased reporter activity after co-transfection of the wild-type mTOR 3′UTR plasmid with miR-100 into 293T cells. c Protein expression of mTOR visualized by western blotting. GAPDH served as an internal control. d Protein semi-quantitative analysis of mTOR. Data are presented as mean ± SEM (n = 3). Statistical analysis was performed using one-way ANOVA followed by Tukey’s test; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.001