Table 3 Primary and secondary antibodies used in this study
Cat. No | Company | Antibody | Species | Dilution |
|---|---|---|---|---|
Antibodies used for immunofluorescence | ||||
SC-5279 | Santa Cruz Biotechnology | OCT4 | Mouse | 1/200 |
AF1997 | R&D | NANOG | Goat | 1/100 |
MAB4303 | Millipore | SSEA-3 | Mouse | 1/200 |
MAB4360 | Millipore | TRA-1-60 | Mouse | 1/200 |
SC-1237 | Santa Cruz Biotechnology | GATA4 | Goat | 1/200 |
SC-6554 | Santa Cruz Biotechnology | FOXA2 | Goat | 1/200 |
SC-8987 | Santa Cruz Biotechnology | HNF4α | Rabbit | 1/200 |
M0888 | Dako | CK19 | Mouse | 1/200 |
SC-8399 | Santa Cruz Biotechnology | AFP | Mouse | 1/200 |
A6084 | Sigma | ALB | Mouse | 1/100 |
SC-27639 | Santa Cruz Biotechnology | CYP3A4 | Goat | 1/100 |
A0012 | Dako | A1AT | Rabbit | 1/400 |
187362 | Life Technologies | CLDN1 | Rabbit | 1/200 |
555675 | BD Pharmingen | CD81 | Mouse | 1/200 |
NB400-131 | Novus Biologicals | SRB1 | Goat | 1/200 |
NBP1-85047 | Novus Biologicals | ZO-1 | Rabbit | 1/400 |
AB42488 | Abcam | ASGR | Rabbit | 1/100 |
SC-7258 | Santa Cruz Biotechnology | Connexin 32 | Goat | 1/100 |
M3612 | Dako | E-cadherin | Mouse | 1/100 |
AB28482 | Abcam | SREBP2 | Rabbit | 1/100 |
A12379 | Molecular Probes | F-Actin | Mouse | 1/200 |
AB5790 | Abcam | PAX6 | Rabbit | 1/200 |
AB78078 | Abcam | TUJ1 | Mouse | 1/100 |
AB5694 | Abcam | αSMA | Rabbit | 1/100 |
A11055 - A11057 | Molecular Probes | Anti-goat | Donkey | 1/1000 |
A32306 - A10042 | Molecular Probes | Anti-rabbit | Donkey | 1/1000 |
A21202 - A10037 | Molecular Probes | Anti-mouse | Donkey | 1/1000 |
Antibodies used for western blot | ||||
AB52818 | Abcam | LDLR | Rabbit | 1/500 |
AB8227 | Abcam | Actin | Rabbit | 1/3000 |
NA934V | Thermo Fisher | Anti-Rabbit HRP | Donkey | 1/10000 |
Antibodies used for flow cytometry | ||||
12-8843-42 | eBioscience | SSEA-4 | Mouse | 1/25 |
12-8883-82 | eBioscience | TRA-1-81 | Mouse | 1/25 |
130-109-844 | Miltenyi Biotec | CXCR4 | Mouse | 1/25 |
130-080-301 | Miltenyi Biotec | EPCAM | Mouse | 1/20 |
12-4742-42 | eBioscience | Mouse IgG3-PE | Mouse | 1/25 |
12-5890-82 | eBioscience | Mouse IgM-PE | Mouse | 1/25 |
130-091-836 | Miltenyi Biotec | Mouse IgG2a-APC | Mouse | 1/25 |
A07795 | Beckman Coulter | Mouse IgG1-FITC | Mouse | 1/20 |