Fig. 4

Wnt1 was the target gene of miR-140-5p. a Luciferase reporter gene detection results suggested that the luciferase activity of the mimic group was significantly lower than that of the mimic NC group. b Western blot analysis showed the expressions of Wnt1 and total β-catenin proteins were the highest in the inhibitor group and the lowest in the mimic group; the differences among the control, mimic NC, and inhibitor NC groups were not statistically significant. c Western blot analysis showed the expression of nuclear β-catenin protein was the highest in the inhibitor group and the lowest in the mimic group; the differences among the control, mimic NC, and inhibitor NC groups were not statistically significant. *vs. the control group, P < 0.05; ^#vs. the mimic NC group, P < 0.05; ^@vs. the inhibitor NC group, P < 0.05; ^&vs. the mimic group, P < 0.05. n = 6