Stem Cell Research & Therapy

Table 1 Primer sequences of real-time RT-PCR

From: iRoot BP Plus promotes osteo/odontogenic differentiation of bone marrow mesenchymal stem cells via MAPK pathways and autophagy

Genes	Primers	Sequences (5′–3′)
Osx	Forward	GGAGGCACAAAGAAGCCATA
Osx	Reverse	GGGAAAGGGTGGGTAGTCAT
Alp	Forward	GGAACGGATCTCGGGGTACA
Alp	Reverse	ATGAGTTGGTAAGGCAGGGT
Runx2	Forward	TTAACGTCAGCAGGAGCAG
Runx2	Reverse	CTTCACCCCCAGGACCAAG
Opn	Forward	ATCTGCCGACGTACCCTTTC
Opn	Reverse	TCGTGGCTCTGATGTTCCAG
Dspp	Forward	ATCTGCCGACGTACCCTTTC
Dspp	Reverse	CCTCCTGCGTGTATCCCATC
Gapdh	Forward	CACTGAGCATCTCCCTCACAA
Gapdh	Reverse	GTATTCGAGAGAAGGGAGGGCT

Osx osterix, Alp alkaline phosphatase, Runx2 Runt-related transcription factor 2, Opn osteopontin, Dspp dentin sialophosphoprotein, Gapdh D-gluteraldehyde-3-phosphate dehydrogenase

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ISSN: 1757-6512

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