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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: From 3D to 3D: isolation of mesenchymal stem/stromal cells into a three-dimensional human platelet lysate matrix

Fig. 1

Migration and evaluation of cells from adipose tissue after cryopreservation of whole adipose tissue with and without a cryoprotective medium and further embedding in into PLMatrix. a Scheme of embedding adipose tissue into PLMatrix for 3D explant isolation of MSCs. b Micrographs showing the outgrowth of MSCs from the adipose tissue into PLMatrix after thawing of adipose tissue without and with cryomedia at different time points. c Cell number, d viability, and e metabolic activity assessed with resazurin-based TOX-8 assay of MSCs after outgrowth from the adipose tissue cryopreserved without and with cryomedia into PLMatrix after 14 days of outgrowth. Data represented as average ± SD from n = 3 experiments, *p < 0.05

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Stem Cell Research & Therapy

ISSN: 1757-6512