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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: From 3D to 3D: isolation of mesenchymal stem/stromal cells into a three-dimensional human platelet lysate matrix

Fig. 2

Comparison of the isolation of MSCs from whole adipose tissue embedded into 3D PLMatrix or via 2D explant culture. a Histological sections stained with hematoxylin (blue, stains for nuclei) and eosin (pink, stains for collagen) of adipose tissue embedded in 3D PLMatrix hydrogel with MSCs migrating into the hydrogel after 11 days. b Outgrowth of MSCs from adipose tissue into 3D PLMatrix or on a 2D plastic surface (2D explant) after 7, 11, and 14 days of culture. c Amount of MSCs harvested from 3D PLMatrix or 2D plastic surface after 7, 11, and 14 days of outgrowth from adipose tissue. d Calcein-AM and PI and e DAPI stain of MSCs in 3D PLMatrix and on 2D plastic surface on day 14 after outgrowth from adipose tissue. Data represented as average ± SD from n = 3 experiments, *p < 0.05

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Stem Cell Research & Therapy

ISSN: 1757-6512