Fig. 3

Characterization of cells that migrated from adipose tissue into PLMatrix. a Surface marker analysis of MSCs from standard enzymatic isolation procedure (2D control), isolation from tissue explants on a 2D plastic surface (2D explant), and isolation from tissue explants in the 3D PLMatrix. b Average x-fold expression of surface markers of phenotype normalized to isotype control. c Micrographs of cells cultivated in an adipogenic, chondrogenic, or osteogenic differentiation medium for 21 days, stained with Oil Red O, Alcian blue, and Alizarin Red, respectively. Data of flow cytometric analysis represents ≥ 10.000 gated events