Fig. 1
Mixed chimerism with MHC-mismatched, but not MHC-matched BM transplants prevents T1D in NOD mice. Wild-type NOD mice were injected i.v. with anti-CD3/CD8 Abs on days − 8 and − 3. On day 0, the conditioned mice were injected i.v. with CD4+ T cell-depleted spleen cells (20 × 106 each) from MHC-mismatched B6 or MHC-matched H-2g7 B6 mice. The control mice were given anti-CD3/CD8 conditioning only. Diabetes development was monitored by blood glucose analysis for up to 100 days after BMT. On day 100, the pancreas, spleen, BM, and thymus were harvested from the mice. a Diabetes development curve after BMT. b Statistical analysis of the percentages of insulitis. a, b The data were pooled from three independent experiments (4–5 mice per group in each experiment). c Representative FACS profile of spleen cells showing the percentages of donor (CD45.2+) or host (CD45.2−) T cells (TCR+) and B cells (B220+). d Representative FACS profile of BM cells showing the percentages of donor (CD45.2+) or host (CD45.2−) B cells (B220+). e Gated donor (CD45.2+) or host (CD45.1+) thymocytes were shown in CD4 versus CD8. The percentages of CD4+CD8+ DP thymocytes are shown