Fig. 5

MHC-mismatched mESC-TEP-transplanted mice do not have an upregulated PD-1 and downregulated IL-7Rα expression on host-type peripheral T cells. Wild-type NOD mice were injected i.v. with anti-CD3/CD8 Abs on days − 8 and − 3 as in Fig. 1. On day 0, the conditioned mice were injected i.v. with BM and CD4⁺ T cell-depleted spleen cells (20 × 10⁶ each) from MHC-matched H-2^g7 B6 mice or MHC-mismatched B6 mice as in Fig. 1. A group of the MHC-matched BMT recipients was also injected i.t. with B6 mESC-EpCAM1⁺ TEPs (5 × 10⁴) as in Fig. 2. On day 100, the expression of PD-1, IL-7Rα, FAS and TIM3 by residual splenic host-type T cells (CD45.1⁺CD4⁺TCRβ⁺) was analyzed by flow cytometry. The Expression levels of a PD-1, b IL-7Rα, c FAS and d TIM3. One representative FACS profile and mean fluorescence (n = 4–6) are shown. *P < 0.05, vs MHC-matched BM transplants